The nonpregnant group was chosen at random from the local healthy female population, and the rest of the study subjects were sampled by drawing 5 ml of venous blood from the elbow in a test tube before outpatient or inpatient intravenous treatment. The specimen was centrifuged at 3500 r/min for 15 min at room temperature after standing for 30 min, and the supernatant was collected and kept at -80 °C until analysis. According to the manufacturer’s protocol, serum Cat C and D levels were measured using Cat C and D ELISA kits (HB2902Hu, HS2902-Hu; HB022-Hu, HS022-Hu; Shanghai Hengyuan Biotechnology LTD), and final results were expressed as pg/mL.
