【文献标题】Genistein alleviates chronic heat stress-induced lipid metabolism disorder and mitochondrial energetic dysfunction by activating the GPR30-AMPK-PGC-1a signaling pathways in the livers of broiler chickens
The levels of serum lipids, including high-density lipoprotein cholesterol (HDL-C, Cat. A112-1-1), TC, TG, and low-density lipoprotein cholesterol (LDL-C, Cat. A113-1-1), were determined by commercial kits obtained from Nanjing Jiancheng Biotechnology Institution (Nanjing, China). The levels of serum hormones, including insulin (INS, Cat. HB030-Ch), triiodothyronine (T3, Cat. HB079-Ch), corticosterone (CORT, Cat. HB098-Ch), and thyroxine (T4, Cat. HB137-Ch), were determined by ELISA kits procured from Shanghai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The measurements were performed following the manufacturer’s instructions.