【文献标题】Efect of angiotensin II on irradiation exacerbated decompression sickness
【作者】Jie Fu Fan, Yang KaiWang, Min Liu,et.al
【作者单位】第二军医大学（Second Military Medical University）
【文献中引用产品】
大鼠白介素1β (IL-1β)ELISA试剂盒
大鼠白介素6(IL-6)ELISA试剂盒
大鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒
大鼠血管紧张素II（ANGII）ELISA试剂盒
大鼠血管紧张素转化酶（ACE）ELISA试剂盒
大鼠活性氧（ROS）ELISA试剂盒
【关键词】
【DOI】https://doi.org/10.1038/s41598-023-38752-z
【影响因子(IF)】4.6
【出版期刊】《Scientific Reports》
【产品原文引用】
Lung tissues were removed from the rats in each group afer symptom observation for 30 min afer decompression treatment. An enzyme-linked immunosorbent assay (ELISA) was used to detect ACE enzyme activity, Ang II concentration, and reactive oxygen species (ROS) level in collected lung tissue to the protocol (ELISA Kit for ACE, HB1258-Ra, Henyuan Biotechnology, China), (ELISA Kit for Ang II, HB193-Ra, Henyuan Biotechnology, China), (Reactive Oxygen Species Assay Kit, P-846-SH, Henyuan Biotechnology, China).
Rats were anaesthetized with urethane (800 mg/kg i.p.) and a-chloralose (40 mg/kg i.p.) afer symptom observation. Bronchoalveolar lavage was then performed as in previous studies27,28. Briefy, bronchoalveolar lavage fuid (BALF) was obtained using a syringe flled with 2 ml of 0.9% saline, which was repeated three times, and the total volume recovered was more than 80%. Te lavage fuid was centrifuged at 1200 rpm for 10 min. An ELISA was used to detect interleukin-1β (IL-1β), IL-6, TNF-α, and Ang II concentration in collected BALF to the protocol (ELISA Kit for IL-1β, HS972-Ra, Henyuan Biotechnology, China), (ELISA Kit for IL-6, HS959-Ra, Henyuan Biotechnology, China), (ELISA Kit for TNF-α, HS044-Ra, Henyuan Biotechnology, China).
