In this study, single cell gel electrophoresis (SCGE) and 8-OHdG content detection were used to evaluate the effect of fosthiazate on DNA damage in E. fetida. The SCGE refers to the method of Li et al. [29]. The detailed steps are shown in the Supplementary Text S3. For 8-OHdG, the E. fetida 8-OHdG ELISA kit (Hengyuan, Shanghai, China) was used to calculate its content by measuring absorbance at 450 nm.
