Inflammatory factors in the colorectal tissues of each mouse were determined. Briefly, the 0.1 g tissues were homogenized and centrifuged, and then the supernatant was taken for detection. The commercial enzyme-linked immunosorbent assay (ELISA) kits were respectively used to measure the contents of IL-1, IL-6, TNF-α and IFN-γ according to the manufacturer's manuals (Shanghai Hengyuan Biotech. Co., Ltd,
