【文献标题】Effects of mannan oligosaccharides (MOS) on glucose and lipid metabolism of largemouth bass (Micropterus salmoides) fed with high carbohydrate diet
The glucose and triglyceride (TG) in serum, liver glycogen and hydroxyproline were determined by using specific commercial assay kits (Jiancheng Biotech Co., China) according to manufacturer’s protocols. The serum insulin and glucagon-like peptide 1 (GLP-1) were measured by Enzyme-linked immunosorbent assay (ELISA) kits (Shanghai Hengyuan Biological Technology Co.,Ltd, China) according to manufacturer’s instructions. The activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), and phosphoenolpyruvate carboxykinase (PEPCK) were measured by using commercial kits (Jiancheng Biotech Co., China) according to the relevant kit protocols. The activities of glucose-6-phosphatase (G6pase) and fructose-1,6-diphosphate (FBPase) were assessed by commercial kits (Shanghai Hengyuan Biological Technology Co.,Ltd, China) according to the manufacturer’s methods.
