【文献标题】PTX3 alleviates hard metal-induced acute lung injury through potentiating efferocytosis
【作者】Weiwen Yan , Dongyu Ma , Yi Liu ,et.al
【作者单位】南京医科大学( Nanjing Medical University)
【文献中引用产品】
小鼠白介素1β (IL-1β)ELISA试剂盒
【关键词】Hard metal lung disease ,PTX3 ,Apoptosis ,Efferocytosis ,Inflammation
【DOI】https://doi.org/10.1016/j.ecoenv.2021.113139
【影响因子(IF)】7.12
【出版期刊】《Ecotoxicology and Environmental Safet》
【产品原文引用】
2.17. Enzyme-linked immunosorbent assay (ELISA)
The supernatant of THP-1 macrophages was collected after WC-Co (150 μg/ml) treatment. Levels of IL-1β were measured using ELISA
kits according to the manufacturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd, China). The absorbance was detected by a
microplate reader at the wavelength of 450 nm (Bio-Tek EL, USA), and the cytokines concentration of each example was analyzed according to the standard curve of IL-1β.