【文献标题】Dietary Glucose Ameliorates Impaired Intestinal Development and Immune Homeostasis Disorders Induced by Chronic Cold
Stress in Pig Model
【作者】Guodong Sun , Xin Song , Yingbin Zou,et.al
【作者单位】东北农业大学（Northeast Agricultural University）
【文献中引用产品】
猪D-乳酸(D-LA)ELISA试剂盒
猪γ干扰素(IFN-γ)ELISA试剂盒
猪分泌型免疫球蛋白A(SIgA)ELISA试剂盒
【关键词】chronic cold stress; small intestinal mucosa; TLR4/MyD88 signal pathway; pyroptosis;glucose
【DOI】https://doi.org/10.3390/ijms23147730
【影响因子(IF)】6.2
【出版期刊】《International Journal of Molecular Scienc》
【产品原文引用】
Determination of plasma D-lactate concentration using ELISA kit as per manufacturer guidelines of the kit (Shanghai Hengyuan Biological, Shanghai, China).
For the measurement of intestinal inflammation and immune factors concentrations, a 10% tissue homogenate of jejunal and ileal mucosa was produced using saline [59]. The total protein concentration was calculated using the BCA Protein Assay Kit (Beyotime Biotechnology, Nantong, China). The concentrations of IFN-γ and sIgA in jejunal mucosa and ileal mucosa were measured using an ELISA kit per the manufacturer’s instructions (Shanghai Hengyuan Biological, Shanghai, China).
