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恒远产品文献:微生物α-Glu,ATP,F420,CAT,AK引用文献
发布时间:2023/4/26 9:16:29 阅读次数:302

【文献标题】Upgrade the high-load anaerobic digestion and relieve acid stress through the strategy of side-stream micro-aeration: biochemical performances, microbial response and intrinsic mechanisms

【作者】Wen Li , Yongli Liu , Baocun Wu , et.al
【作者单位】重庆大学 Chongqing University
【文献中引用产品】
微生物α葡萄糖苷酶(α-Glu)ELISA试剂盒
微生物三磷酸腺苷(ATP)ELISA试剂盒
微生物辅酶F420(F420)ELISA试剂盒
微生物过氧化氢酶(CAT)ELISA试剂盒 
微生物乙酸激酶(AK)ELISA试剂盒
【关键词】High-load anaerobic digestion ,Acid stress ,Side-stream micro-aeration ,Metagenomic analysis 
【DOI】https://doi.org/10.1016/j.watres.2022.118850
【影响因子(IF)】13.39
出版期刊】《Water Research》
【产品原文引用】
Measurements of total solid (TS), volatile solid (VS), chemical oxygen demand (COD), alkalinity and NH4 +-N were conducted in accordance 
with standard methods (Gao et al., 2022). pH was determined by pH meter (FE28, METTLER TOLEDO, Switzerland). Gasbags were used to 
collect the biogas in the digesters, and the volume was measured by a graduated syringe through repeat extracting the gas from the bag. The 
biogas composition and volatile fatty acids (VFAs) were determined by a gas chromatography (Jiedao GC1690, Hangzhou). The operation process of gas chromatography was introduced in detail in previous study (Tian et al., 2021). α-Glucosidase activity, ATP content, coenzyme F420, CAT (catalase), as well as acetate kinase were measured by using the ELISA kit (Shanghai hengyuan biological technology co., LTD). Electron transfer activity (ETS) was measured using the method reported by Wan et al. (2016). Quantification of C, H and O elements using an elemental analyzer (Elementar Analysensysteme GmbH, Unicube). Confocal laser scanning microscope (CLSM) staining and observation was conducted according to the method reported by Chen et al. (2007). 

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