【文献标题】Hepatic Stellate Cells Secreted Hepatocyte Growth Factor Contributes to the Chemoresistance of Hepatocellular Carcinoma
【作者】Guofeng Yu, Yingying Jing, Xingrui Kou, et.al
【作者单位】上海第二军医大学（Second Military Medical University, Shanghai）
【文献中引用产品】
人肝细胞生长因子(HGF)ELISA试剂盒
人转化生长因子β（TGF-β）ELISA试剂盒
【DOI】doi:10.1371/journal.pone.0073312
【影响因子(IF)】3.23
【出版期刊】《PLOS ONE》
【产品原文引用】
Conditioned medium and ELISA
Cultured media (CM) were collected from LX-2 cells cultured in 2D culture dishes and centrifuged at 1000g for 5 minutes to obtain the supernatant. LX-2 CM was incubated with HGF antibody (GTX10678, 0.5 μg/ml) for 24h at 4oC to obtain HGFdepleted LX-2 CM. The level of HGF and TGF-β in CM waere determined by using human HGF ELISA kit (HY10158E, Shanghai HengYuan Biological Technology Co., Ltd) and TGF- β ELISA kit (HY10108E, Shanghai HengYuan Biological Technology Co., Ltd) according to manufacturer’s instructions.
