【文献标题】Effect of inorganic phosphate on migration and osteogenic differentiation of bone marrow mesenchymal stem cells
【作者】Hengzhang Lin, Yong Zhou, Qun Lei , et.al
【作者单位】福建医学院(FujianMedical University)
【文献中引用产品】
二水合磷酸二氢钠（NaHPO4·2H2O）
二水合磷酸氢二钠（NaH2PO4）2H2O
【关键词】Phosphate, Mesenchymal stem cells, Bone defect, Bone substitute materials, Implant
【DOI】https://doi.org/10.1186/s12861-020-00229-x
【影响因子(IF)】1.97
【出版期刊】《BMC Developmental Biology》
【产品原文引用】
To prepare 5 different concentrations of Pi, Sodium dihydrogen phosphate dihydrate (NaHPO4 ? 2H2O;Shanghai Hengyuan Biotechnology Co., Ltd., China) and disodium hydrogen phosphate dihydrate(NaH2PO4 ?2H2O; Shanghai Hengyuan Biotechnology Co., Ltd.,China) were dissolved in HEPES buffer solution at a ratio of 4: 1 [12] to make Pi stock solution concentration of 200, 400, 600, 800, 1000 mM. Pi culture mediums concentration of 2, 4, 6, 8, 10 mM (2mMPi, 4mMPi, 6mMPi, 8mMPi, 10mMPi) were prepared by serially diluting stock solutions in mesenchymal stem cell growth medium (Guangzhou Saiye Biotechnology Co., Ltd., China). hBM-MSCs(HUXMA-01001, Guangzhou Saiye Biotechnology Co., Ltd., China) incubated in mesenchymal stem cell growth medium (GM) were applied as a control.
