【文献标题】Excessive ammonia inhalation causes liver damage and dysfunction by altering gene networks associated with oxidative stress and immune function
【作者】Xiangyin Zeng , Runze Liu , Yutao Li , et.al
【作者单位】东北农业大学（Northeast Agricultural University）
【文献中引用产品】
猪白介素4(IL-4)ELISA试剂盒
猪白介素6(IL-6)ELISA试剂盒
猪白介素8(IL-8)ELISA试剂盒
猪免疫球蛋白G(IgG)ELISA试剂盒
猪免疫球蛋白M(IgM)ELISA试剂盒
【关键词】Ammonia ,Liver damage ,Oxidative stress ,Immune dysfunction ,Gene network
【DOI】https://doi.org/10.1016/j.ecoenv.2021.112203
【影响因子(IF)】6.2
【出版期刊】《Ecotoxicology and Environmental Safety》
【产品原文引用】
2.5. Measurement of immune function
To assess the effect of ammonia exposure on immune function, serum samples were used to analyze cytokine and immunoglobulin levels at Northeast Agricultural University. The cytokines (IL-4, IL-6 and IL-8) were determined by ELISA kits according to the kit instructions (Shanghai Hengyuan Biotechnology Co., Ltd, China). All cytokines were reported in ng/L. Inter and intra assay cytokine CVs were IL-4 (14.67%;< 16%), IL-6 (10.12%; < 13%) and IL-8 (13.1%;< 16%). Serum immunoglobulin-A (IgA), IgG and IgM were detected by ELISA kits using the enzyme-labeled instrument (SpectraMax? ABS00254, California, USA). All immunoglobulin levels were expressed in mg/mL. IgA ELISA kit was purchased from ShangHai Yanjin Biotechnology Co., Ltd (China). IgG and IgM ELISA kits were detected by ELISA kits (Shanghai Hengyuan Biotechnology Co., Ltd, China).
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