Mice blood was collected before sacrificed on the 14th day, as described in a previous study [42].The levels of antigen-specific antibodies (IgG1, IgG2a, IgM) produced by mice in sera samples were determined using commercially available mouse ELISA kits by following the manufacture’s instruction (HengYuan, Shanghai, China). The commercial double antibody sandwich ELISA kits (HengYuan, Shanghai, China) were used to evaluate cytokines’ level (IL-4, IL-12, IL-17, IFN-γ, and TGF-β) produced in the sera of all experimental mice, according to the manufacturer’s instruction.
