恒远产品文献:大鼠睾酮（T）ELISA试剂盒引用文献
发布时间:2021/3/30 16:34:10 阅读次数：771

【文献标题】PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats

【作者】Yang Yang，Yajing Feng，Hui Huang，et.al

【作者单位】郑州大学第一附属医院（The First Affiliated Hospital of Zhengzhou University）

【文献中引用产品】

大鼠睾酮（T）ELISA试剂盒

【关键词】Fine particulate matter，Inositol-requiring enzyme，C-Jun NH 2-terminal kinase，Autophagy，Reproductive injury

【DOI】https://doi.org/10.1016/j.ecoenv.2021.111924

【影响因子(IF)】6.20

【出版期刊】《Ecotoxicology and Environmental Safety》

【产品原文引用】

2.5. Determination of serum testosterone

We evaluated serum testosterone level in rats by ELISA. After the rats were sacrificed, approximately 5 mL of abdominal aortic blood was collected and subjected to centrifugation at 3000 rpm for 15 min, and the serum was carefully aspirated and stored at 20 for later use. According to the instructions for the rat testosterone ELISA kit (Hengyuan Biotech, Shanghai, China), we used different concentrations of testosterone standards and corresponding absorbance values to prepare a standard curve, and thus determined the testosterone in each group of samples after observing the sample absorbance.