恒远产品文献:鱼维生素K1（VK1）ELISA试剂盒引用文献
发布时间:2021/1/18 11:35:07 阅读次数：842

【文献标题】Disruption of Abcc6 Transporter in Zebrafish Causes Ocular Calcification and Cardiac Fibrosis

【作者】Jianjian Sun，Peilu She，Xu Liu，et.al

【作者单位】华东师范大学（East China Normal University）

【文献中引用产品】

鱼维生素K1（VK1）ELISA试剂盒

【关键词】PXE，abcc6，vitamin K，ocular calcification，cardiac fibrosis

【DOI】https://doi.org/10.3390/ijms22010278

【影响因子(IF)】4.55

【出版期刊】《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》

【产品原文引用】

4.12. ELISA Assay

This kit used the double antibody sandwich method to determine the level of vitamin K1 (VK1) in fish (Shanghai Hengyuan Biological Technology Co., Ltd., H-42702, Shanghai,China). The monoclonal antibody vitamin K1 was added into the micropore and combined with horseradish peroxidase (HRP) to form a complex. After polyester, tetramethylbenzidine (TMB) was added to develop color. Under TMB catalysis, it was transformed to blue. There was a positive correlation between the color and the fish vitamin K1 in the fish,measured at 450 nm by enzyme-linked immunosorbent assay. The absorbance (OD value)of fish vitamin K1 in the sample was calculated by the standard curve. To determine the cMGP levels, a human matrix gamma carboxyglutamic acid protein (cMGP) detection kit was selected (Shanghai Hengyuan Biological Technology Co., Ltd., H-11621, Shanghai,China). The purified HRP labeled cMGP antibody was coated on the microporous plate to form a complex. After thorough washing, the substrate TMB was added for color rendering.The experiment was repeated three times.