【文献标题】Prenatal nicotine exposure intergenerationally programs imperfect articular cartilage via histone deacetylation through maternal lineage
【作者】Zhe Xie, Zhe Zhao, Xu Yang，et.al
【作者单位】武汉大学中南医院（Zhongnan Hospital of Wuhan University）
【文献中引用产品】
【关键词】Nicotine prenatal exposure; Articular cartilage; Transforming growth factor β; Histone
【DOI】doi:10.1016/j.taap.2018.03.018
【影响因子(IF)】3.94
【出版期刊】《Toxicology and Applied Pharmacology》
【产品原文引用】
Chemicals and reagents
Nicotine (CAS no. 54-11-5) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Safranin O (CAS no. 477-73-6) and fast green (CAS no. 2353-45-9) were purchased from Hengyuan Biotech (HengYuan Biological Technology Co., Ltd, Shanghai, China). Trizol reagent kits were obtained from Omega BioTek (Doraville, GA, USA). Exscript RT reagent kit and SYBR(R) Premix Ex TaqTM were obtained from TaKaRa Biotechnology (Dalian, China). Oligonucleotide primers for analyzed genes were synthesized by Sangon Biotech (Shanghai, China). DMEM/F12 (1:1) and fetal bovine serum were supplied by Gibco (St. Louis, MO, USA). Polyclonal antibodies for COL2A1, SOX9, TGFβ, TGFβ receptor 1 (TGFβR1) and Smad2/3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Other chemicals and agents were of analytical grade.
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