【文献标题】Tumor-associated macrophages promote cancer stem cell-like 4 properties via transforming growth factor-beta1-induced 5 epithelial–mesenchymal transition in hepatocellular carcinoma
【作者】Qing-Min Fan,Ying-Ying Jing,Guo-Feng Yu,et.al
【作者单位】第二军医大学(The Second Military Medical University)
【文献中引用产品】
【关键词】Hepatocellular carcinoma,Tumor-associated macrophages,Cancer stem cells,Epithelial to mesenchymal transition,Transforming growth factor-beta1
【DOI】http://dx.doi.org/10.1016/j.canlet.2014.05.008
【影响因子(IF)】6.50
【出版期刊】《Cancer Letters》
【产品原文引用】
20 ng/ml IL-4 and 100 ng/ml LPS plus 20 ng/ml IFN-c was used to stimulate two types of macrophages for 24 h, respectively. Then the supernatants of RAW264.7 cells were centrifuged and collected. Amounts of TGF-b1 secreted by macrophage was measured using commercial ELISA kits (Shanghai Hengyuan Biotechnology Co., Ltd.), according to the manufacturer’s instructions.
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