【出版期刊】《International Journal of Molecular Sciences》
【产品原文引用】
The samples, mixed with eight females or males per repeat, were crushed and homogenized in the Phosphate Buffered Saline (PBS) solution (0.01 M, pH 7.4). Three repeat samples were used for each treatment. The homogenate was centrifuged at 5000 rpm/min for 10 min and the supernatant was separated. The collected supernatant was then stored at 80 C. The levels of the total PGs, PGE2, PGD2, 11β-13,14-dihydro-15-keto PGF2α (PGFM), 5-iPF2a-VI, and ROS were detected by commercial ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions. These kits employed HRP-conjugate mouse antibodies to quantify PGs and ROS levels in samples. Statistical differences were subjected to one-way analysis of variance (ANOVA), followed by Tukey’s multiple comparison test. The statistical significance level was set at p < 0.05.
