To quantify AOX activity in postharvest strawberry samples under different treatments, 1.0 g of white fleshed strawberry sample was ground with liquid nitrogen and homogenized in phosphate buffered saline (PBS, pH 7.2) buffer. After 1 h incubation on an ice bath, the extracts were centrifuged at 8000 × g for 15 min at 4 C and the supernatant was collected for ELISA quantification. Then, AOX activity was assayed according to the instructions of Plant AOX activity ELISA kit (Shanghai Hengyuan Biotechnology Co., Ltd.).
