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恒远产品文献:昆虫P450s,CarE,GST,ELISA试剂盒引用文献
发布时间:2024/3/15 9:24:34 阅读次数:115

【文献标题】Effects of sublethal concentrations of cyantraniliprole on the biology and metabolic enzyme activities of Laodelphax striatellus (Fall′en) 

【作者】Xueting Wang , Xiang Liu , Chang Liu , et.al
【作者单位】山东农业大学(Shandong Agricultural University)
【文献中引用产品】
昆虫P450胆固醇侧链裂解酶(P450s)ELISA试剂盒 酶活
昆虫羧酸酯酶(CarE)ELISA试剂盒  酶活
昆虫谷胱甘肽S转移酶(GST)ELISA试剂盒 酶活
【关键词】Cyantraniliprole ,Laodelphax striatellus ,Life table ,Sublethal effect 
【DOI】https://doi.org/10.1016/j.cropro.2022.105964
【影响因子(IF)】3.03
出版期刊】《Crop Protection》
【产品原文引用】
The activities of P450s, CarE, and GST were examined using an ELISA kit (Shanghai Hengyuan Biotechnology Co., Ltd., Shanghai, China) following the manufacturer’s instructions. The diluted standard substance and reagents in the kit and enzyme protein were added to a 96-well plate according to the manufacturer’s instructions. After the reaction had finished, the OD value at 450 nm was determined using a microplate reader. A standard curve was drawn according to the concentrations of the standard substance and the corresponding OD values, and then the enzyme activities were calculated based on the standard curve. 

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